alcian blue solution  (Servicebio Inc)

Journal: Gut Microbes

Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

doi: 10.1080/19490976.2026.2662638

Figure Lengend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions.

Techniques: Staining, Western Blot, Quantitative RT-PCR, Expressing

Journal: Frontiers in Pharmacology

Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

doi: 10.3389/fphar.2026.1803816

Figure Lengend Snippet: V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

doi: 10.3389/fphar.2026.1803816

Figure Lengend Snippet: V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

Techniques: Staining, Western Blot